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Phospho-Stathmin 1-S25 polyclonal antibody

货号: BS74135

价格：￥ 1680

货期：现货（1-2个工作日）

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No. :BS74135

Product Name :Phospho-Stathmin 1-S25 polyclonal antibody

Swiss-Prot :P16949

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:100

Background :This gene belongs to the stathmin family of genes. It encodes a ubiquitous cytosolic phosphoprotein proposed to function as an intracellular relay integrating regulatory signals of the cellular environment. The encoded protein is involved in the regulation of the microtubule filament system by destabilizing microtubules. It prevents assembly and promotes disassembly of microtubules. Multiple transcript variants encoding different isoforms have been found for this gene.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Phosphorylated

BiowMW :17KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :STMN1;C1orf215;LAP18;Lag;OP18;PP17;PP19;PR22;SMN;stathmin

Immunogen :A synthetic phosphorylated peptide around S25 of human Stathmin 1(NP_005554.1).

Conjugate :Unconjugated

Modification :Phosphorylated

Western blot analysis of various lysates, using Phospho-Stathmin 1-S25 antibody at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 60s.

Immunohistochemistry of paraffin-embedded human breast carcinoma using Phospho-Stathmin 1-S25 antibody .Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.