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KPNA4 polyclonal antibody

货号: BS7413

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS7413

Product Name :KPNA4 polyclonal antibody

Swiss-Prot :O00629

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC, IF/ICC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200|IF/ICC,1:50 - 1:200

Background :The nuclear import of karyophilic proteins is directed by short amino acid sequences termed nuclear localization signals (NLSs). Karyopherins, or importins, are cytoplasmic proteins that recognize NLSs and dock NLS-containing proteins to the nuclear pore complex. The protein encoded by this gene shares the sequence similarity with Xenopus importin-alpha and Saccharomyces cerevisiae Srp1. This protein is found to interact with the NLSs of DNA helicase Q1 and SV40 T antigen.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :58kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :KPNA4;IPOA3;QIP1;SRP3

Immunogen :Recombinant fusion protein of human KPNA4(NP_002259.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using KPNA4 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

Immunohistochemistry of paraffin-embedded rat heart using KPNA4 Antibody at dilution of 1:200 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded human esophageal cancer using KPNA4 Antibody at dilution of 1:200 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunofluorescence analysis of U2OS cells using KPNA4 antibody . Blue: DAPI for nuclear staining.