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Pan DiMethyl-lysine polyclonal antibody

货号: BS74115

价格：￥ 1680

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS74115

Product Name :Pan DiMethyl-lysine polyclonal antibody

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB

Application_all :WB,1:500 - 1:2000

Background :The lysine methylation chemical reaction is the reversible addition of one, two or three methyl groups to the ε-nitrogen of a lysine side chain, forming monomethylated, dimethylated and trimethylated derivatives (referred to here as ‘Kme1’, ‘Kme2’ and ‘Kme3’).

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Methylated

BiowMW :17-48KDa

Note :For research use only, not for use in diagnostic procedure.

Immunogen :A synthetic peptide corresponding to a sequence containing dimethylatedK.

Conjugate :Unconjugated

Modification :Methylated

Western blot analysis of extracts of various cell lines, using Pan DiMethyl-lysine Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 3min.