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MonoMethyl-UHRF1-K385 polyclonal antibody

货号: BS74109

价格：￥ 1680

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS74109

Product Name :MonoMethyl-UHRF1-K385 polyclonal antibody

Swiss-Prot :Q96T88

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :DB, WB, IHC

Application_all :DB,1:500 - 1:2000|WB,1:500 - 1:2000|IHC,1:50 - 1:200

Background :This gene encodes a member of a subfamily of RING-finger type E3 ubiquitin ligases. The protein binds to specific DNA sequences, and recruits a histone deacetylase to regulate gene expression. Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1/S transition by regulating topoisomerase IIalpha and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint. It is regarded as a hub protein for the integration of epigenetic information. This gene is up-regulated in various cancers, and it is therefore considered to be a therapeutic target. Multiple transcript variants encoding different isoforms have been found for this gene. A related pseudogene exists on chromosome 12.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Methylated

BiowMW :91kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :UHRF1;ICBP90;Np95;RNF106;TDRD22;hNP95;hUHRF1;huNp95

Immunogen :A synthetic monomethylated peptide around K385 of human UHRF1(NP_001041666.1).

Conjugate :Unconjugated

Modification :Methylated

Western blot analysis of extracts of C6 cells, using MonoMethyl-UHRF1-K385 antibody at 1:1000 dilution.C6 cells were treated by MG132 at 37℃ for 90 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit .
Exposure time: 1s.

Dot-blot analysis of all sorts of methylation peptides using MonoMethyl-UHRF1-K385 antibody at 1:1000 dilution.