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Cyclin D2 polyclonal antibody

货号: BS7404

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS7404

Product Name :Cyclin D2 polyclonal antibody

Swiss-Prot :P30279

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC, IF/ICC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200|IF/ICC,1:50 - 1:200

Background :The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with CDK4 or CDK6 and functions as a regulatory subunit of the complex, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. Knockout studies of the homologous gene in mouse suggest the essential roles of this gene in ovarian granulosa and germ cell proliferation. High level expression of this gene was observed in ovarian and testicular tumors. Mutations in this gene are associated with megalencephaly-polymicrogyria-polydactyly-hydrocephalus syndrome 3 (MPPH3).

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :33KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :CCND2;KIAK0002;MPPH3;cyclin D2;Cyclin D2

Immunogen :Recombinant fusion protein of human Cyclin D2(NP_001750.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of RD, using Cyclin D2 antibody at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit .
Exposure time: 60s.

Immunofluorescence analysis of U2OS cells using Cyclin D2 antibody . Blue: DAPI for nuclear staining.

miR-206 is down-regulated in breast cancer and inhibits cell proliferation through the up-regulation of cyclinD2

JournalBiochemical And Biophysical Research Communications

ApplicationIHC

IF2.985

SpeciesHuman

PMID
MicroRNA-99a induces G1-phase cell cycle arrest and suppresses tumorigenicity in renal cell carcinoma

JournalBmc Cancer

ApplicationWB

IF2.933

SpeciesMouse

PMID
Downregulation of prostaglandin E2 is involved in hindgut mucosal damage in lactating goats fed a high‐concentrate diet

JournalExperimental Physiology

ApplicationWB

IF2.431

SpeciesGoat

PMID26593381