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Acetyl-Histone H4-K12 polyclonal antibody

货号: BS74026

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS74026

Product Name :Acetyl-Histone H4-K12 polyclonal antibody

Swiss-Prot :P62805

Host :Rabbit

Reactivity :Human, Mouse, Rat, Other (Wide Range)

Applications :WB, IHC, IF/ICC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200|IF/ICC,1:50 - 1:200

Background :Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Acetylated Antibodies

BiowMW :11KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :HIST1H4B;H4/I;H4FI;histone H4;Histone H4;HIST1H4A;HIST2H4A;FO108;H4;H4/n;H4F2;H4FN;HIST2H4

Immunogen :A synthetic acetylated peptide around K12 of human Histone H4(NP_001029249.1).

Conjugate :Unconjugated

Modification :Acetylated

Western blot analysis of extracts of various cell lines, using Acetyl-Histone H4-K12 antibody at 1:500 dilution.HeLa cells and C6 cells were treated by TSA at 37℃ for 18 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 30s.

Immunohistochemistry of paraffin-embedded human appendix using Acetyl-Histone H4-K12 antibody at dilution of 1:200 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded rat ovary using Acetyl-Histone H4-K12 antibody at dilution of 1:200 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded mouse testis using Acetyl-Histone H4-K12 antibody at dilution of 1:200 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.