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PSMB1 polyclonal antibody

货号: BS7397
价格 1140
货期 现货(1-2个工作日)
25ul 50ul 100ul 200ul
No. :BS7397
Product Name :PSMB1 polyclonal antibody
Swiss-Prot :P20618
Host :Rabbit
Reactivity :Human, Mouse, Rat
Applications :WB, IF/ICC
Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200
Background :The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is tightly linked to the TBP (TATA-binding protein) gene in human and in mouse, and is transcribed in the opposite orientation in both species.
Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :Polyclonal Antibodies
BiowMW :25kDa
Note :For research use only, not for use in diagnostic procedure.
Alternative Name :PSMB1;HC5;PMSB1;PSC5
Immunogen :Recombinant fusion protein of human PSMB1(NP_002784.1).
Conjugate :Unconjugated
Modification :Unmodification
Western blot analysis of extracts of various cell lines, using PSMB1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 1s.
Immunofluorescence analysis of C6 cells using PSMB1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of L929 cells using PSMB1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using PSMB1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

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400-660-9091

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com