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ANGPTL4 polyclonal antibody

货号: BS7349

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS7349

Product Name :ANGPTL4 polyclonal antibody

Swiss-Prot :Q9BY76

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC, IF/ICC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200|IF/ICC,1:50 - 1:200

Background :This gene encodes a glycosylated, secreted protein containing a C-terminal fibrinogen domain. The encoded protein is induced by peroxisome proliferation activators and functions as a serum hormone that regulates glucose homeostasis, lipid metabolism, and insulin sensitivity. This protein can also act as an apoptosis survival factor for vascular endothelial cells and can prevent metastasis by inhibiting vascular growth and tumor cell invasion. The C-terminal domain may be proteolytically-cleaved from the full-length secreted protein. Decreased expression of this gene has been associated with type 2 diabetes. Alternative splicing results in multiple transcript variants. This gene was previously referred to as ANGPTL2 but has been renamed ANGPTL4.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :45-50KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :ANGPTL4;ARP4;FIAF;HARP;HFARP;NL2;PGAR;TGQTL;UNQ171;pp1158

Immunogen :Recombinant fusion protein of human ANGPTL4(NP_647475.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of A-549, using ANGPTL4 antibody at 1:650 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 5s.

Western blot analysis of Rat lung, using ANGPTL4 antibody at 1:650 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 180s.

Immunohistochemistry of paraffin-embedded rat kidney using ANGPTL4 Rabbit pAb at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunofluorescence analysis of NIH/3T3 cells using ANGPTL4 Rabbit pAb at dilution of 1:50 . Blue: DAPI for nuclear staining.