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GPX4 polyclonal antibody

货号: BS7323

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS7323

Product Name :GPX4 polyclonal antibody

Swiss-Prot :P36969

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC, IF/ICC

Application_all :WB,1:500 - 1:1000|IHC,1:50 - 1:200|IF/ICC,1:50 - 1:200

Background :The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of hydrogen peroxide, organic hydroperoxides and lipid hydroperoxides, and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme has a high preference for lipid hydroperoxides and protects cells against membrane lipid peroxidation and cell death. It is also required for normal sperm development; thus, it has been identified as a 'moonlighting' protein because of its ability to serve dual functions as a peroxidase, as well as a structural protein in mature spermatozoa. Mutations in this gene are associated with Sedaghatian type of spondylometaphyseal dysplasia (SMDS). This isozyme is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Alternatively spliced transcript variants have been found for this gene.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :GPX4 Polyclonal Antibody detects endogenous levels of GPX4 protein.

BiowMW :22KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :GPX4;GPx-4;GSHPx-4;MCSP;PHGPx;SMDS;snGPx;snPHGPx

Immunogen :KLH-conjugated synthetic peptide encompassing a sequence within the center region of human GPX4.

Conjugate :Unconjugated

Modification :Unmodified

Western blot analysis of GPX4 expression in HEK293T (A), K562 (B) whole cell lysates.

Western blot analysis of extracts of Mouse spleen, using HMGCR Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 10s.

Immunohistochemistry of paraffin-embedded human small intestine using HMGCR antibody at dilution of 1:200 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded human uterine cancer using HMGCR antibody at dilution of 1:200 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Asiatic acid alleviates LPS-induced acute kidney injury in broilers by inhibiting oxidative stress and ferroptosis via activation of the Nrf2 pathway

JournalFOOD AND CHEMICAL TOXICOLOGY

ApplicationWB,IF

IF5.572

SpeciesChicken

PMID36244460
Lycium barbarum polysaccharides ameliorate canine acute liver injury by reducing oxidative stress, protecting mitochondrial function, and regulating metabolic pathways

JournalJournal of Zhejiang University-SCIENCE B

ApplicationWB

IF5.552

SpeciesCanine

PMID36751701
Soybean Lecithin–Gallic Acid Complex Sensitizes Lung Cancer Cells to Radiation Through Ferroptosis Regulated by Nrf2/SLC7A11/GPX4 Pathway

JournalNutrients

ApplicationWB

IF4.8

SpeciesHuman

PMID40219018