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TFAM polyclonal antibody

货号: BS7319

价格：￥ 1140

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :BS7319

Product Name :TFAM polyclonal antibody

Swiss-Prot :Q00059

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC, IF/ICC, ChIP

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200|IF/ICC,1:50 - 1:200|ChIP,1:50 - 1:200

Background :This gene encodes a key mitochondrial transcription factor containing two high mobility group motifs. The encoded protein also functions in mitochondrial DNA replication and repair. Sequence polymorphisms in this gene are associated with Alzheimer's and Parkinson's diseases. There are pseudogenes for this gene on chromosomes 6, 7, and 11. Alternative splicing results in multiple transcript variants.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :25KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :TFAM;MTDPS15;MTTF1;MTTFA;TCF6;TCF6L1;TCF6L2;TCF6L3

Immunogen :A synthetic peptide of human TFAM(NP_003192.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using TFAM antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 1s.

Immunohistochemistry of paraffin-embedded rat brain using TFAM antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded human colon using TFAM antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunofluorescence analysis of NIH/3T3 cells using TFAM Rabbit pAb at dilution of 1:100 . Blue: DAPI for nuclear staining.

In ovo injection of betaine alleviates corticosterone-induced fatty liver in chickens through epigenetic modifications

JournalScientific Reports

ApplicationWB

IF4.011

SpeciesChicken

PMID28059170
Effect of gomisin A on osteoblast differentiation in high glucose-mediated oxidative stress

JournalPhytomedicine

ApplicationWB

IF5.34

SpeciesMouse

PMID31790903