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BRD3 polyclonal antibody

货号: BS72924

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS72924

Product Name :BRD3 polyclonal antibody

Swiss-Prot :Q15059

Host :Rabbit

Reactivity :Human, Rat

Applications :WB, IHC, IF/ICC

Application_all :WB,1:500 - 1:1000|IHC,1:50 - 1:100|IF/ICC,1:50 - 1:100

Background :This gene was identified based on its homology to the gene encoding the RING3 protein, a serine/threonine kinase. The gene localizes to 9q34, a region which contains several major histocompatibility complex (MHC) genes. The function of the encoded protein is not known.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :115kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :BRD3;ORFX;RING3L

Immunogen :Recombinant fusion protein of human BRD3(NP_031397.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using BRD3 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 5s.

Immunohistochemistry of paraffin-embedded rat testis using BRD3 antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.