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PXR polyclonal antibody

货号: BS7284
价格 1140
货期 现货(1-2个工作日)
25ul 50ul 100ul 200ul
No. :BS7284
Product Name :PXR polyclonal antibody
Swiss-Prot :O75469
Host :Rabbit
Reactivity :Human, Mouse, Rat
Applications :WB, IF/ICC, IP
Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200|IP,1:50 - 1:200
Background :This gene product belongs to the nuclear receptor superfamily, members of which are transcription factors characterized by a ligand-binding domain and a DNA-binding domain. The encoded protein is a transcriptional regulator of the cytochrome P450 gene CYP3A4, binding to the response element of the CYP3A4 promoter as a heterodimer with the 9-cis retinoic acid receptor RXR. It is activated by a range of compounds that induce CYP3A4, including dexamethasone and rifampicin. Several alternatively spliced transcripts encoding different isoforms, some of which use non-AUG (CUG) translation initiation codon, have been described for this gene. Additional transcript variants exist, however, they have not been fully characterized.
Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :Polyclonal Antibodies
BiowMW :50KDa
Note :For research use only, not for use in diagnostic procedure.
Alternative Name :NR1I2;BXR;ONR1;PAR;PAR1;PAR2;PARq;PRR;PXR;SAR;SXR
Immunogen :Recombinant fusion protein of human PXR(NP_003880.3).
Conjugate :Unconjugated
Modification :Unmodification
Western blot analysis of extracts of various cell lines, using PXR antibody at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 60s.
Immunofluorescence analysis of C6 cells using PXR Polyclonal Antibody at dilution of 1:100 . Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 600ug extracts of Mouse stomach cells using 3ug PXR antibody . Western blot was performed from the immunoprecipitate using PXR antibody at a dilution of 1:500.

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400-660-9091

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com