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HNF4G polyclonal antibody

货号: BS72827

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS72827

Product Name :HNF4G polyclonal antibody

Swiss-Prot :Q14541

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:100

Background :Transcription factor. Has a lower transcription activation potential than HNF4-alpha.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :50kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :HNF4G;NR2A2;NR2A3

Immunogen :Recombinant fusion protein of human HNF4G(NP_004124.4).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using HNF4G antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 15s.

Immunofluorescence analysis of U-2 OS cells using HNF4G Polyclonal Antibody at dilution of 1:100 . Blue: DAPI for nuclear staining.