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TRIM4 polyclonal antibody

货号: BS72805

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS72805

Product Name :TRIM4 polyclonal antibody

Swiss-Prot :Q9C037

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

Background :The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic bodies. Its function has not been identified. Alternatively spliced transcript variants that encode different isoforms have been described.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :50-60KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :TRIM4;RNF87

Immunogen :Recombinant fusion protein of human TRIM4(NP_149082.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using TRIM4 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 30s.

Immunofluorescence analysis of NIH/3T3 cells using TRIM4 Rabbit pAb at dilution of 1:250 . Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC-12 cells using TRIM4 Rabbit pAb at dilution of 1:250 . Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U2OS cells using TRIM4 Rabbit pAb at dilution of 1:250 . Blue: DAPI for nuclear staining.