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DDX31 polyclonal antibody

货号: BS72797

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS72797

Product Name :DDX31 polyclonal antibody

Swiss-Prot :Q9H8H2

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

Background :DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a member of this family. The function of this member has not been determined. Alternative splicing of this gene generates multiple transcript variants encoding different isoforms.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :94kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :DDX31;PPP1R25

Immunogen :Recombinant fusion protein of human DDX31(NP_073616.6).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of A-549 cells, using DDX31 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit .
Exposure time: 3min.

Immunofluorescence analysis of HeLa cells using DDX31 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of L929 cells using DDX31 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.