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LARS polyclonal antibody

货号: BS72681

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS72681

Product Name :LARS polyclonal antibody

Swiss-Prot :Q9P2J5

Host :Rabbit

Reactivity :Human, Mouse

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

Background :This gene encodes a cytosolic leucine-tRNA synthetase, a member of the class I aminoacyl-tRNA synthetase family. The encoded enzyme catalyzes the ATP-dependent ligation of L-leucine to tRNA(Leu). It is found in the cytoplasm as part of a multisynthetase complex and interacts with the arginine tRNA synthetase through its C-terminal domain. A mutation in this gene was found in affected individuals with infantile liver failure syndrome 1. Alternatively spliced transcript variants of this gene have been observed.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :134kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :LARS;HSPC192;ILFS1;LARS1;LEURS;LEUS;LFIS;LRS;PIG44;RNTLS;hr025Cl

Immunogen :Recombinant fusion protein of human LARS(NP_064502.9).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using LARS antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 60s.

Immunofluorescence analysis of L929 cells using LARS Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U2OS cells using LARS Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.