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EGLN1/EGLN2 polyclonal antibody

货号: BS72635

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS72635

Product Name :EGLN1/EGLN2 polyclonal antibody

Swiss-Prot :Q9GZT9/Q96KS0

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB

Application_all :WB,1:500 - 1:2000

Background :The proteins encoded by EGLN1/EGLN2 gene catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. HIF is a transcriptional complex that plays a central role in mammalian oxygen homeostasis. This protein functions as a cellular oxygen sensor, and under normal oxygen concentration, modification by prolyl hydroxylation is a key regulatory event that targets HIF subunits for proteasomal destruction via the von Hippel-Lindau ubiquitylation complex.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :44kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :EGLN1/EGLN2

Immunogen :A synthetic peptide of human EGLN1/EGLN2(NP_071334.1/NP_444274.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using EGLN1/EGLN2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 90s.