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SMPD4 polyclonal antibody

货号: BS72607

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS72607

Product Name :SMPD4 polyclonal antibody

Swiss-Prot :Q9NXE4

Host :Rabbit

Reactivity :Human, Mouse

Applications :WB, IF/ICC

Application_all :WB,1:200 - 1:2000|IF/ICC,1:50 - 1:200

Background :The protein encoded by this gene is a sphingomyelinase that catalyzes the hydrolysis of membrane sphingomyelin to form phosphorylcholine and ceramide. This gene is activated by DNA damage, cellular stress, and tumor necrosis factor, but it is downregulated by wild-type p53. The encoded protein localizes to the endoplasmic reticulum and Golgi network.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :100kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :SMPD4;NET13;NSMASE-3;NSMASE3

Immunogen :Recombinant fusion protein of human SMPD4(NP_060421.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of A-549 cells, using SMPD4 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 15s.

Immunofluorescence analysis of L929 cells using SMPD4 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.