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VAV3 polyclonal antibody

货号: BS72583

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS72583

Product Name :VAV3 polyclonal antibody

Swiss-Prot :Q9UKW4

Host :Rabbit

Reactivity :Human, Mouse

Applications :WB, IF/ICC

Application_all :WB,1:200 - 1:2000|IF/ICC,1:50 - 1:200

Background :This gene is a member of the VAV gene family. The VAV proteins are guanine nucleotide exchange factors (GEFs) for Rho family GTPases that activate pathways leading to actin cytoskeletal rearrangements and transcriptional alterations. This gene product acts as a GEF preferentially for RhoG, RhoA, and to a lesser extent, RAC1, and it associates maximally with the nucleotide-free states of these GTPases. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :105kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :VAV3

Immunogen :Recombinant fusion protein of human VAV3(NP_006104.4).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using VAV3 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 2s.

Immunofluorescence analysis of HeLa cells using VAV3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of L929 cells using VAV3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.