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ADAMTS1 polyclonal antibody

货号: BS72575

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS72575

Product Name :ADAMTS1 polyclonal antibody

Swiss-Prot :Q9UHI8

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

Background :This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motif) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The protein encoded by this gene contains two disintegrin loops and three C-terminal TS motifs and has anti-angiogenic activity. The expression of this gene may be associated with various inflammatory processes as well as development of cancer cachexia. This gene is likely to be necessary for normal growth, fertility, and organ morphology and function.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :105kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :ADAMTS1;C3-C5;METH1

Immunogen :Recombinant fusion protein of human ADAMTS1(NP_008919.3).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using ADAMTS1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 1s.

Immunofluorescence analysis of 293F cells using ADAMTS1 Rabbit pAb at dilution of 1:25 . Blue: DAPI for nuclear staining.

Immunofluorescence analysis of HeLa cells using ADAMTS1 Rabbit pAb at dilution of 1:25 . Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using ADAMTS1 Rabbit pAb at dilution of 1:25 . Blue: DAPI for nuclear staining.