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SIM2 polyclonal antibody

货号: BS72529

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS72529

Product Name :SIM2 polyclonal antibody

Swiss-Prot :Q14190

Host :Rabbit

Reactivity :Human

Applications :WB, IHC

Application_all :WB,1:500 - 1:2000|IHC,1:100 - 1:200

Background :This gene represents a homolog of the Drosophila single-minded (sim) gene, which encodes a transcription factor that is a master regulator of neurogenesis. The encoded protein is ubiquitinated by RING-IBR-RING-type E3 ubiquitin ligases, including the parkin RBR E3 ubiquitin protein ligase. This gene maps within the so-called Down syndrome chromosomal region, and is thus thought to contribute to some specific Down syndrome phenotypes. Alternative splicing of this gene results in multiple transcript variants.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :56kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :SIM2;HMC13F06;HMC29C01;SIM;bHLHe15

Immunogen :Recombinant fusion protein of human SIM2(NP_033664.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using SIM2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 5s.