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Asymmetric DiMethyl-Histone H3-R17 polyclonal antibody

货号: BS7247

价格：￥ 1350

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

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No. :BS7247

Product Name :Asymmetric DiMethyl-Histone H3-R17 polyclonal antibody

Swiss-Prot :P68431

Host :Rabbit

Reactivity :Human, Mouse, Rat, Other (Wide Range)

Applications :WB, IF/ICC, IP

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200|IP,1:50 - 1:200

Background :Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Methylated Antibodies

BiowMW :17KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :HIST1H3J;H3/j;H3FJ

Immunogen :A synthetic asymmetric dimethylated peptide around R17 of human histone H3(NP_003520.1).

Conjugate :Unconjugated

Modification :Methylated

Western blot analysis of extracts of various cell lines, using Asymmetric DiMethyl-Histone H3-R17 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 90s.

Dot-blot analysis of all sorts of methylation peptides using Asymmetric DiMethyl-Histone H3-R17 antibody .

Immunofluorescence analysis of 293T cells using Asymmetric DiMethyl-Histone H3-R17 antibody . Blue: DAPI for nuclear staining.

Immunofluorescence analysis of HeLa cells using Asymmetric DiMethyl-Histone H3-R17 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.