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VPS26A polyclonal antibody

货号: BS72451

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS72451

Product Name :VPS26A polyclonal antibody

Swiss-Prot :O75436

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

Background :This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. Alternative splicing results in multiple transcript variants encoding different isoforms.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :40KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :VPS26A;HB58;Hbeta58;PEP8A;VPS26

Immunogen :Recombinant fusion protein of human VPS26A(NP_004887.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of various lysates, using VPS26A antibody at 1:413 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 180s.

Immunofluorescence analysis of NIH-3T3 cells using VPS26A Polyclonal Antibody at dilution of 1:100 . Blue: DAPI for nuclear staining.