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GBP2 polyclonal antibody

货号: BS72425

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS72425

Product Name :GBP2 polyclonal antibody

Swiss-Prot :P32456

Host :Rabbit

Reactivity :Human, Mouse

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

Background :This gene belongs to the guanine-binding protein (GBP) family, which includes interferon-induced proteins that can bind to guanine nucleotides (GMP, GDP and GTP). The encoded protein is a GTPase which hydrolyzes GTP, predominantly to GDP. The protein may play a role as a marker of squamous cell carcinomas.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :67kDa/70kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :GBP2

Immunogen :Recombinant fusion protein of human GBP2(NP_004111.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using GBP2 antibody at 1:3000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit ._Exposure time: 90s.

Western blot analysis of extracts of 293T cells, using GBP2 antibody at 1:1000 dilution.293T cells treated with different concentrations of IFNA1.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit .
Exposure time: 10s.

Immunofluorescence analysis of L929 cells using GBP2 Rabbit pAb at dilution of 1:100 . Blue: DAPI for nuclear staining.