Chromatin immunoprecipitations were performed with cross-linked chromatin from K-562 cells and DiMethyl-Histone H3-K79 Rabbit pAb . The ChIP sequencing results indicate the enrichment pattern of DiMethyl-Histone H3-K79 in selected genomic region and representative gene loci , as shown in figure.
Western blot analysis of extracts of HeLa cells, using DiMethyl-Histone H3-K79 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 90s.
Dot-blot analysis of all sorts of methylation peptides using DiMethyl-Histone H3-K79 antibody .
Immunohistochemistry of paraffin-embedded human mammary cancer using DiMethyl-Histone H3-K79 antibody at dilution of 1:200 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.