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C/EBPB polyclonal antibody

货号: BS72318

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS72318

Product Name :C/EBPB polyclonal antibody

Swiss-Prot :P17676

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC

Application_all :WB,1:500 - 1:1000|IHC,1:20 - 1:100

Background :This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain. The encoded protein functions as a homodimer but can also form heterodimers with CCAAT/enhancer-binding proteins alpha, delta, and gamma. Activity of this protein is important in the regulation of genes involved in immune and inflammatory responses, among other processes. The use of alternative in-frame AUG start codons results in multiple protein isoforms, each with distinct biological functions.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :48KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :C/EBP-beta;IL6DBP;NF-IL6;TCF5;CEBP beta;CEBPB

Immunogen :Recombinant fusion protein of human C/EBPB(NP_005185.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of various lysates, using C/EBPB antibody at 1:740 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 1s.

Immunohistochemistry of paraffin-embedded rat liver using C/EBPB Rabbit pAb at dilution of 1:100 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded human breast cancer using C/EBPB Rabbit pAb at dilution of 1:100 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

TGF-β/BMP signaling pathway is involved in cerium-promoted osteogenic differentiation of mesenchymal stem cells

JournalJournal Of Cellular Biochemistry

ApplicationWB

IF4.429

SpeciesMouse

PMID23150386
Ursolic acid induces HL60 monocytic differentiation and upregulates C/EBPβ expression by ERK pathway activation

JournalAnti-Cancer Drugs

ApplicationWB

IF2.248

SpeciesHuman

PMID20948428
Adipose progenitor cells reside among the mature adipocytes: morphological research using an organotypic culture system

JournalCell Biology International

ApplicationIHC

IF2.571

SpeciesRat

PMID26095163