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KDM3A polyclonal antibody

货号: BS72270

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

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No. :BS72270

Product Name :KDM3A polyclonal antibody

Swiss-Prot :Q9Y4C1

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC, IF/ICC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200|IF/ICC,1:50 - 1:200

Background :This gene encodes a zinc finger protein that contains a jumonji domain and may play a role in hormone-dependent transcriptional activation. Alternative splicing results in multiple transcript variants.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :147KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :KDM3A;JHDM2A;JHMD2A;JMJD1;JMJD1A;TSGA

Immunogen :A synthetic peptide of human KDM3A(NP_060903.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of Mouse testis, using KDM3A antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit .
Exposure time: 180s.

Immunohistochemistry of paraffin-embedded human liver cancer using KDM3A Rabbit pAb at dilution of 1:150 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunofluorescence analysis of U2OS cells using KDM3A Rabbit pAb at dilution of 1:150 . Blue: DAPI for nuclear staining.