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ITPR3 polyclonal antibody

货号: BS72246

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

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No. :BS72246

Product Name :ITPR3 polyclonal antibody

Swiss-Prot :Q14573

Host :Rabbit

Reactivity :Human, Mouse

Applications :WB, IF/ICC, IP

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200|IP,1:50 - 1:200

Background :This gene encodes a receptor for inositol 1,4,5-trisphosphate, a second messenger that mediates the release of intracellular calcium. The receptor contains a calcium channel at the C-terminus and the ligand-binding site at the N-terminus. Knockout studies in mice suggest that type 2 and type 3 inositol 1,4,5-trisphosphate receptors play a key role in exocrine secretion underlying energy metabolism and growth.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :300kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :ITPR3;IP3R;IP3R3

Immunogen :Recombinant fusion protein of human ITPR3(NP_002215.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using ITPR3 antibody at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 90s.

Immunofluorescence analysis of NIH-3T3 cells using ITPR3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 300ug extracts of Hela cells using 3ug ITPR3 antibody . Western blot was performed from the immunoprecipitate using ITPR3 antibody at a dilution of 1:1000.

ITPR3 facilitates tumor growth, metastasis and stemness by inducing the NF-ĸB/CD44 pathway in urinary bladder carcinoma

JournalJournal Of Experimental & Clinical Cancer Research

ApplicationWB

IF7.068

SpeciesHuman

PMID33573671