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[KO Validated] MTCH2 polyclonal antibody

货号: BS72216

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS72216

Product Name :[KO Validated] MTCH2 polyclonal antibody

Swiss-Prot :Q9Y6C9

Host :Rabbit

Reactivity :Human, Mouse

Applications :WB, IHC, IF/ICC

Application_all :WB,1:1000 - 1:3000|IHC,1:50 - 1:200|IF/ICC,1:50 - 1:200

Background :This gene encodes a member of the SLC25 family of nuclear-encoded transporters that are localized in the inner mitochondrial membrane. Members of this superfamily are involved in many metabolic pathways and cell functions. Genome-wide association studies in human have identified single-nucleotide polymorphisms in several loci associated with obesity. This gene is one such locus, which is highly expressed in white adipose tissue and adipocytes, and thought to play a regulatory role in adipocyte differentiation and biology. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. A recent study showed this gene to be an authentic stop codon readthrough target, and that its mRNA can give rise to an additional C-terminally extended isoform by use of an alternative in-frame translation termination codon.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :33KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :MTCH2;HSPC032;MIMP;SLC25A50

Immunogen :Recombinant fusion protein of human MTCH2(NP_055157.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts from wild type and MTCH2 knockout HeLa cells, using MTCH2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 3s.

Immunofluorescence analysis of NIH-3T3 cells using MTCH2 Polyclonal Antibody at dilution of 1:100 . Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using MTCH2 Polyclonal Antibody at dilution of 1:100 . Blue: DAPI for nuclear staining.