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SYNE3 polyclonal antibody

货号: BS72153

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS72153

Product Name :SYNE3 polyclonal antibody

Swiss-Prot :Q6ZMZ3

Host :Rabbit

Reactivity :Human, Mouse

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

Background :As a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton complex involved in the connection between the nuclear lamina and the cytoskeleton. The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning. Probable anchoring protein which tethers the nucleus to the cytoskeleton by binding PLEC which can associate with the intermediate filament system. Plays a role in the regulation of aortic epithelial cell morphology, and is required for flow-induced centrosome polarization and directional migration in aortic endothelial cells.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :112kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :SYNE3;C14orf139;C14orf49;KASH3;LINC00341;NCRNA00341;NET53;Nesp3;nesprin-3

Immunogen :Recombinant fusion protein of human SYNE3(NP_689805.3).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using SYNE3 antibody at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 90s.

Immunofluorescence analysis of HeLa cells using SYNE3 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using SYNE3 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.