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GCC2 polyclonal antibody

货号: BS72117

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS72117

Product Name :GCC2 polyclonal antibody

Swiss-Prot :Q8IWJ2

Host :Rabbit

Reactivity :Human, Mouse

Applications :WB, IHC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200

Background :The protein encoded by this gene is a peripheral membrane protein localized to the trans-Golgi network. It is sensitive to brefeldin A. This encoded protein contains a GRIP domain which is thought to be used in targeting. Alternative splicing results in multiple transcript variants.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :196kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :GCC2;GCC185;RANBP2L4;REN53

Immunogen :Recombinant fusion protein of human GCC2(NP_852118.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using GCC2 antibody at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 90s.

Immunohistochemistry of paraffin-embedded human colon using GCC2 antibody at dilution of 1:150 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded human stomach using GCC2 antibody at dilution of 1:150 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded human appendix using GCC2 antibody at dilution of 1:150 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.