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[KO Validated] PDK1/PDPK1 polyclonal antibody

货号: BS7193

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS7193

Product Name :[KO Validated] PDK1/PDPK1 polyclonal antibody

Swiss-Prot :O15530

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200

Background :PDPK1 (3-phosphoinositide dependent protein kinase 1) is a protein-coding gene. GO annotations related to this gene include protein serine/threonine kinase activity and insulin receptor binding.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :58-68KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :PDPK1;PDK1;PDPK2;PDPK2P;PRO0461

Immunogen :Recombinant fusion protein of human PDK1/PDPK1(NP_002604.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using PDK1/PDPK1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 90s.

Western blot analysis of extracts from normal and PDK1/PDPK1 knockout HCT116 cells, using PDK1/PDPK1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 90s.

Immunohistochemistry of paraffin-embedded rat brain using PDPK1 antibody at dilution of 1:200 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.