产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

RASGRP1 polyclonal antibody

货号: BS71758

价格：￥ 1140

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS71758

Product Name :RASGRP1 polyclonal antibody

Swiss-Prot :O95267

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC

Application_all :WB,1:1000 - 1:2000|IF/ICC,1:50 - 1:200

Background :This gene is a member of a family of genes characterized by the presence of a Ras superfamily guanine nucleotide exchange factor (GEF) domain. It functions as a diacylglycerol (DAG)-regulated nucleotide exchange factor specifically activating Ras through the exchange of bound GDP for GTP. It activates the Erk/MAP kinase cascade and regulates T-cells and B-cells development, homeostasis and differentiation. Alternatively spliced transcript variants encoding different isoforms have been identified. Altered expression of the different isoforms of this protein may be a cause of susceptibility to systemic lupus erythematosus (SLE).

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :90KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :RASGRP1;CALDAG-GEFI;CALDAG-GEFII;RASGRP;V;hRasGRP1

Immunogen :Recombinant fusion protein of human RASGRP1(NP_005730.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of THP-1 cells, using RASGRP1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 30s.

Immunofluorescence analysis of A-549 cells using RASGRP1 Rabbit pAb at dilution of 1:100 . Blue: DAPI for nuclear staining.

Immunofluorescence analysis of C6 cells using RASGRP1 Rabbit pAb at dilution of 1:100 . Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using RASGRP1 Rabbit pAb at dilution of 1:100 . Blue: DAPI for nuclear staining.