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ADAM10 polyclonal antibody

货号: BS71726

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS71726

Product Name :ADAM10 polyclonal antibody

Swiss-Prot :O14672

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

Background :Members of the ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. This gene encodes and ADAM family member that cleaves many proteins including TNF-alpha and E-cadherin. Alternate splicing results in multiple transcript variants encoding different proteins that may undergo similar processing.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :90KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :ADAM10;AD10;AD18;CD156c;CDw156;HsT18717;MADM;RAK;kuz

Immunogen :Recombinant fusion protein of human ADAM10(NP_001101.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of RAW264.7, using ADAM10 antibody at 1:1740 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit .
Exposure time: 20s.

Immunofluorescence analysis of HeLa cells using ADAM10 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.