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CISD1 polyclonal antibody

货号: BS71677

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS71677

Product Name :CISD1 polyclonal antibody

Swiss-Prot :Q9NZ45

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC

Application_all :WB,1:1000 - 1:2000|IF/ICC,1:50 - 1:200

Background :This gene encodes a protein with a CDGSH iron-sulfur domain and has been shown to bind a redox-active [2Fe-2S] cluster. The encoded protein has been localized to the outer membrane of mitochondria and is thought to play a role in regulation of oxidation. Genes encoding similar proteins are located on chromosomes 4 and 17, and a pseudogene of this gene is located on chromosome 2.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :16KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :CISD1;C10orf70;MDS029;ZCD1;mitoNEET

Immunogen :Recombinant fusion protein of human CISD1(NP_060934.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of various lysates, using CISD1 antibody at 1:1420 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 1s.

Immunofluorescence analysis of NIH/3T3 cells using CISD1 Rabbit pAb at dilution of 1:150 . Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC-12 cells using CISD1 Rabbit pAb at dilution of 1:150 . Blue: DAPI for nuclear staining.