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TST polyclonal antibody

货号: BS71551

价格：￥ 1140

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

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说明书 MSDS SHIP

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No. :BS71551

Product Name :TST polyclonal antibody

Swiss-Prot :Q16762

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC, IP

Application_all :WB,1:1000 - 1:2000|IHC,1:50 - 1:200|IP,1:50 - 1:100

Background :This is one of two neighboring genes encoding similar proteins that each contain two rhodanese domains. The encoded protein is localized to the mitochondria and catalyzes the conversion of thiosulfate and cyanide to thiocyanate and sulfite. In addition, the protein interacts with 5S ribosomal RNA and facilitates its import into the mitochondria. Alternative splicing results in multiple transcript variants.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :36kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :TST;RDS

Immunogen :Recombinant fusion protein of human TST(NP_003303.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using TST antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 5s.

Immunohistochemistry of paraffin-embedded human liver using TST Rabbit pAb at dilution of 1:200 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded human thyroid cancer using TST Rabbit pAb at dilution of 1:200 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunoprecipitation analysis of 200ug extracts of A-549 cells, using 3 ug TST antibody . Western blot was performed from the immunoprecipitate using TST antibody at a dilution of 1:1000.