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EXOSC2 polyclonal antibody

货号: BS71535

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

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No. :BS71535

Product Name :EXOSC2 polyclonal antibody

Swiss-Prot :Q13868

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

Background :Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs, and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :33kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :EXOSC2;RRP4;Rrp4p;hRrp4p;p7

Immunogen :Recombinant fusion protein of human EXOSC2(NP_055100.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using EXOSC2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 5s.

Immunofluorescence analysis of U2OS cells using EXOSC2 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of L929 cells using EXOSC2 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of H9C2 cells using EXOSC2 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.