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ZO-1 polyclonal antibody

货号: BS71522

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

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No. :BS71522

Product Name :ZO-1 polyclonal antibody

Swiss-Prot :Q07157

Host :Rabbit

Reactivity :Human, Mouse

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

Background :This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. Alternative splicing of this gene results in multiple transcript variants.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :250KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :TJP1;ZO-1

Immunogen :Recombinant fusion protein of human TJP1(NP_003248.3).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of HeLa, using ZO-1 antibody at 1:2250 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 30s.

Immunofluorescence analysis of MCF7 cells using ZO-1 Rabbit pAb at dilution of 1:150 . Blue: DAPI for nuclear staining.

Retinoic acid mitigates the NSC319726-induced spermatogenesis dysfunction through cuproptosis-independent mechanisms

JournalCELL BIOLOGY AND TOXICOLOGY

ApplicationWB

IF6.1

SpeciesMouse

PMID38691186