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RPS24 polyclonal antibody

货号: BS71508

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS71508

Product Name :RPS24 polyclonal antibody

Swiss-Prot :P62847

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC, IP

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200|IP,1:50 - 1:100

Background :Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S24E family of ribosomal proteins. It is located in the cytoplasm. Multiple transcript variants encoding different isoforms have been found for this gene. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Mutations in this gene result in Diamond-Blackfan anemia.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :19kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :RPS24;DBA3;S24

Immunogen :Recombinant fusion protein of human RPS24(NP_148982.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using RPS24 antibody at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 30s.

Immunohistochemistry of paraffin-embedded rat brain using RPS24 Rabbit pAb at dilution of 1:200 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug RPS24 antibody . Western blot was performed from the immunoprecipitate using RPS24 antibody at a dilution of 1:1000.