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RAB13 polyclonal antibody

货号: BS71490

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS71490

Product Name :RAB13 polyclonal antibody

Swiss-Prot :P51153

Host :Rabbit

Reactivity :Human, Rat

Applications :WB, IF/ICC

Application_all :WB,1:1000 - 1:2000|IF/ICC,1:50 - 1:200

Background :This gene is a member of the Rab family of small G proteins and plays a role in regulating membrane trafficking between trans-Golgi network (TGN) and recycling endosomes (RE). The encoded protein is involved in the assembly of tight junctions, which are components of the apical junctional complex (AJC) of epithelial cells. The AJC plays a role in forming a barrier between luminal contents and the underlying tissue. Additional functions associated with the protein include endocytic recycling of occludin, regulation of epithelial cell scattering, neuronal regeneration and regulation of neurite outgrowth. Alternately spliced transcript variants have been observed for this gene. A pseudogene associated with this gene is located on chromosome 12.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :23kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :RAB13;GIG4

Immunogen :Recombinant fusion protein of human RAB13(NP_002861.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using RAB13 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 1s.

Immunofluorescence analysis of U-2 OS cells using RAB13 Rabbit pAb at dilution of 1:100 . Blue: DAPI for nuclear staining.