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SMMHC/MYH11 polyclonal antibody

货号: BS71479

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS71479

Product Name :SMMHC/MYH11 polyclonal antibody

Swiss-Prot :P35749

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

Background :The protein encoded by this gene is a smooth muscle myosin belonging to the myosin heavy chain family. The gene product is a subunit of a hexameric protein that consists of two heavy chain subunits and two pairs of non-identical light chain subunits. It functions as a major contractile protein, converting chemical energy into mechanical energy through the hydrolysis of ATP. The gene encoding a human ortholog of rat NUDE1 is transcribed from the reverse strand of this gene, and its 3' end overlaps with that of the latter. The pericentric inversion of chromosome 16 [inv(16)(p13q22)] produces a chimeric transcript that encodes a protein consisting of the first 165 residues from the N terminus of core-binding factor beta in a fusion with the C-terminal portion of the smooth muscle myosin heavy chain. This chromosomal rearrangement is associated with acute myeloid leukemia of the M4Eo subtype. Alternative splicing generates isoforms that are differentially expressed, with ratios changing during muscle cell maturation. Alternatively spliced transcript variants encoding different isoforms have been identified.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :250KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :MYH11;AAT4;FAA4;SMHC;SMMHC;myosin-11

Immunogen :Recombinant fusion protein of human SMMHC/SMMHC/MYH11(NP_001035203.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using SMMHC/MYH11 antibody at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 1s.

Immunofluorescence analysis of human placenta cells using SMMHC/SMMHC/MYH11 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.