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[KO Validated] DNAJA1 polyclonal antibody

货号: BS71474

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS71474

Product Name :[KO Validated] DNAJA1 polyclonal antibody

Swiss-Prot :P31689

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

Background :This gene encodes a member of the DnaJ family of proteins, which act as heat shock protein 70 cochaperones. Heat shock proteins facilitate protein folding, trafficking, prevention of aggregation, and proteolytic degradation. Members of this family are characterized by a highly conserved N-terminal J domain, a glycine/phenylalanine-rich region, four CxxCxGxG zinc finger repeats, and a C-terminal substrate-binding domain. The J domain mediates the interaction with heat shock protein 70 to recruit substrates and regulate ATP hydrolysis activity. In humans, this gene has been implicated in positive regulation of virus replication through co-option by the influenza A virus. Several pseudogenes of this gene are found on other chromosomes.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :45KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :DNAJA1;DJ-2;DjA1;HDJ2;HSDJ;HSJ-2;HSJ2;HSPF4;NEDD7;hDJ-2

Immunogen :Recombinant fusion protein of human DNAJA1(NP_001530.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts from wild type and DNAJA1 knockout 293T cells, using DNAJA1 antibody at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 1s.

Immunofluorescence analysis of NIH/3T3 cells using DNAJA1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.