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FBXW11 polyclonal antibody

货号: BS71445

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS71445

Product Name :FBXW11 polyclonal antibody

Swiss-Prot :Q9UKB1

Host :Rabbit

Reactivity :Human

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:100

Background :This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbws class and, in addition to an F-box, contains multiple WD40 repeats. This gene contains at least 14 exons, and its alternative splicing generates 3 transcript variants diverging at the presence/absence of two alternate exons.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :62KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :FBXW11;BTRC2;BTRCP2;FBW1B;FBXW1B;Fbw11;Hos

Immunogen :Recombinant fusion protein of human FBXW11(NP_036432.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of A-431 cells, using FBXW11 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 90s.

Immunofluorescence analysis of A-431 cells using FBXW11 Rabbit pAb at dilution of 1:200 . Blue: DAPI for nuclear staining.

Immunofluorescence analysis of HeLa cells using FBXW11 Rabbit pAb at dilution of 1:200 . Blue: DAPI for nuclear staining.