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Ikaros polyclonal antibody

货号: BS7141

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS7141

Product Name :Ikaros polyclonal antibody

Swiss-Prot :Q13422

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC, ChIP

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200|ChIP,1:50 - 1:200

Background :This gene encodes a transcription factor that belongs to the family of zinc-finger DNA-binding proteins associated with chromatin remodeling. The expression of this protein is restricted to the fetal and adult hemo-lymphopoietic system, and it functions as a regulator of lymphocyte differentiation. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene. Most isoforms share a common C-terminal domain, which contains two zinc finger motifs that are required for hetero- or homo-dimerization, and for interactions with other proteins. The isoforms, however, differ in the number of N-terminal zinc finger motifs that bind DNA and in nuclear localization signal presence, resulting in members with and without DNA-binding properties. Only a few isoforms contain the requisite three or more N-terminal zinc motifs that confer high affinity binding to a specific core DNA sequence element in the promoters of target genes. The non-DNA-binding isoforms are largely found in the cytoplasm, and are thought to function as dominant-negative factors. Overexpression of some dominant-negative isoforms have been associated with B-cell malignancies, such as acute lymphoblastic leukemia (ALL).

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :50-70KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :IKZF1;CVID13;Hs.54452;IK1;IKAROS;LYF1;LyF-1;PPP1R92;PRO0758;ZNFN1A1

Immunogen :A synthetic peptide of human Ikaros(NP_006051.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of Raji cells, using Ikaros antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 30s.

Chromatin immunoprecipitation analysis of extracts of K-562 cells, using Ikaros antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.