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TJP2 polyclonal antibody

货号: BS70945

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS70945

Product Name :TJP2 polyclonal antibody

Swiss-Prot :Q9UDY2

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IP

Application_all :WB,1:500 - 1:2000|IP,1:50 - 1:200

Background :This gene encodes a zonula occluden that is a member of the membrane-associated guanylate kinase homolog family. The encoded protein functions as a component of the tight junction barrier in epithelial and endothelial cells and is necessary for proper assembly of tight junctions. Mutations in this gene have been identified in patients with hypercholanemia, and genomic duplication of a 270 kb region including this gene causes autosomal dominant deafness-51. Alternatively spliced transcripts encoding multiple isoforms have been observed for this gene.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :160kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :TJP2;C9DUPq21.11;DFNA51;DUP9q21.11;PFIC4;X104;ZO2

Immunogen :Recombinant fusion protein of human TJP2(NP_004808.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using TJP2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 90s.

Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug TJP2 antibody . Western blot was performed from the immunoprecipitate using TJP2 antibody at a dilution of 1:1000.