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CD209 polyclonal antibody

货号: BS70885

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS70885

Product Name :CD209 polyclonal antibody

Swiss-Prot :Q9NNX6

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

Background :This gene encodes a transmembrane receptor and is often referred to as DC-SIGN because of its expression on the surface of dendritic cells and macrophages. The encoded protein is involved in the innate immune system and recognizes numerous evolutionarily divergent pathogens ranging from parasites to viruses with a large impact on public health. The protein is organized into three distinct domains: an N-terminal transmembrane domain, a tandem-repeat neck domain and C-type lectin carbohydrate recognition domain. The extracellular region consisting of the C-type lectin and neck domains has a dual function as a pathogen recognition receptor and a cell adhesion receptor by binding carbohydrate ligands on the surface of microbes and endogenous cells. The neck region is important for homo-oligomerization which allows the receptor to bind multivalent ligands with high avidity. Variations in the number of 23 amino acid repeats in the neck domain of this protein are rare but have a significant impact on ligand binding ability. This gene is closely related in terms of both sequence and function to a neighboring gene (GeneID 10332; often referred to as L-SIGN). DC-SIGN and L-SIGN differ in their ligand-binding properties and distribution. Alternative splicing results in multiple variants.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :55KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :CD209;CDSIGN;CLEC4L;DC-SIGN;DC-SIGN1

Immunogen :Recombinant fusion protein of human CD209(NP_066978.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of THP-1 cells, using CD209 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 90s.

Immunofluorescence analysis of U-937 cells using CD209 Rabbit pAb at dilution of 1:100 . Blue: DAPI for nuclear staining.

mTOR-Myc axis drives acinar-to-dendritic cell transition and the CD4 + T cell immune response in acute pancreatitis

JournalCell Death & Disease

ApplicationWB

IF6.304

SpeciesMouse

PMID32488108