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Caspase-3 p17 (D175) polyclonal antibody（KU）

货号: BS7073

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS7073

Product Name :Caspase-3 p17 (D175) polyclonal antibody（KU）

Swiss-Prot :P42574

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB

Application_all :WB: 1:500~1:1000

Background :Caspase 3 (also known as CPP32, YAMA and apopain) is the most extensively studied apoptotic protein among caspase family members. Caspase 3 is synthesized as inactive pro enzyme that is processed in cells undergoing apoptosis by self proteolysis and/or cleavage by other upstream proteases (e.g. Caspases 8, 9 and 10).The processed form of Caspase 3 consists of large (17kD) and small (12kD) subunits which associate to form an active enzyme. Caspase 3 is cleaved at Asp28 - Ser29 and Asp175 - Ser176. The active Caspase 3 proteolytically cleaves and activates other caspases (e.g. Caspases 6, 7 and 9), as well as relevant targets in the cells (e.g. PARP and DFF).

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Caspase-3 p17 (D175) polyclonal antibody detects endogenous levels of the large fragment (17 kDa) of activated caspase-3 resulting from cleavage adjacent to Asp175. This antibody does not recognize full length caspase-3 or other cleaved caspases.

BiowMW :~ 17 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Regulation of Apoptosis,Inhibition of Apoptosis,Mitochondrial Control of Apoptosis,Death Receptor Signaling,

Alternative Name :Caspase-3; Caspase3; CASP-3; Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1; SCA-1; CASP3; CPP32

Immunogen :Synthetic peptide conjugated to KLH derived from within residues 150 - 200 of Human active Caspase 3.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of Caspase-3 p17 (D175) polyclonal antibody at 1:500 dilution Lane1:Hela cell lysate treated with Lps Lane2:Raw264.7 cell lysate treated with Lps Lane3:PC12 cell lysate treated with Lps

Western blot (WB) analysis of Caspase-3 p17 (D175) pAb at 1:500 dilution Lane1:HepG2 whole cell lysate(40ug) Lane2:HepG2 treated with Etoposide (20ng/ml, 20h) whole cell lysate (40ug) Lane3:HepG2 treated with Etoposide (20ng/ml, 20h,λ-ppase) whole cell lysate (40ug)

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