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ALIX / PDCD6IP polyclonal antibody

货号: BS70704

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS70704

Product Name :ALIX / PDCD6IP polyclonal antibody

Swiss-Prot :Q8WUM4

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC

Application_all :WB,1:200 - 1:2000|IF/ICC,1:50 - 1:200

Background :This gene encodes a protein that functions within the ESCRT pathway in the abscission stage of cytokinesis, in intralumenal endosomal vesicle formation, and in enveloped virus budding. Studies using mouse cells have shown that overexpression of this protein can block apoptosis. In addition, the product of this gene binds to the product of the PDCD6 gene, a protein required for apoptosis, in a calcium-dependent manner. This gene product also binds to endophilins, proteins that regulate membrane shape during endocytosis. Overexpression of this gene product and endophilins results in cytoplasmic vacuolization, which may be partly responsible for the protection against cell death. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. Related pseudogenes have been identified on chromosome 15.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :105kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :PDCD6IP;AIP1;ALIX;DRIP4;HP95

Immunogen :Recombinant fusion protein of human ALIX / PDCD6IP(NP_037506.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using ALIX / PDCD6IP antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit .
Exposure time: 3min.

Immunofluorescence analysis of L929 cells using ALIX / PDCD6IP antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-251MG cells using ALIX / PDCD6IP antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Exosomes mediate an epithelial‐mesenchymal transition cascade in retinal pigment epithelial cells: Implications for proliferative vitreoretinopathy

JournalJournal Of Cellular And Molecular Medicine

ApplicationWB

IF4.486

SpeciesHuman

PMID33047885