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DAG1 polyclonal antibody

货号: BS70524

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS70524

Product Name :DAG1 polyclonal antibody

Swiss-Prot :Q14118

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC, IP

Application_all :WB,1:500 - 1:1000|IF/ICC,1:20 - 1:100|IP,1:50 - 1:200

Background :This gene encodes dystroglycan, a central component of dystrophin-glycoprotein complex that links the extracellular matrix and the cytoskeleton in the skeletal muscle. The encoded preproprotein undergoes O- and N-glycosylation, and proteolytic processing to generate alpha and beta subunits. Certain mutations in this gene are known to cause distinct forms of muscular dystrophy. Alternative splicing results in multiple transcript variants, all encoding the same protein.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :43kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :156DAG;A3a;AGRNR;DAG;MDDGA9;MDDGC7;MDDGC9;DAG1

Immunogen :Recombinant fusion protein of human DAG1(NP_004384.4).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using DAG1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 10s.

Immunoprecipitation analysis of 300ug extracts of Hela cells using 3ug DAG1 antibody . Western blot was performed from the immunoprecipitate using DAG1 antibody at a dilution of 1:1000.