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RPL8 polyclonal antibody

货号: BS70429
价格 1140
货期 现货(1-2个工作日)
25ul 50ul 100ul 200ul
No. :BS70429
Product Name :RPL8 polyclonal antibody
Swiss-Prot :P62917
Host :Rabbit
Reactivity :Human, Mouse, Rat
Applications :WB, IHC, IF/ICC
Application_all :WB,1:1000 - 1:4000|IHC,1:50 - 1:200|IF/ICC,1:100 - 1:200
Background :Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L2P family of ribosomal proteins. It is located in the cytoplasm. In rat, the protein associates with the 5.8S rRNA, very likely participates in the binding of aminoacyl-tRNA, and is a constituent of the elongation factor 2-binding site at the ribosomal subunit interface. Alternatively spliced transcript variants encoding the same protein exist. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :Polyclonal Antibodies
BiowMW :28kDa
Note :For research use only, not for use in diagnostic procedure.
Alternative Name :RPL8;L8
Immunogen :Recombinant fusion protein of human RPL8(NP_000964.1).
Conjugate :Unconjugated
Modification :Unmodification
Western blot analysis of extracts of various cell lines, using RPL8 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 5s.
Immunohistochemistry of paraffin-embedded rat brain using RPL8 Rabbit pAb at dilution of 1:50 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded mouse brain using RPL8 Rabbit pAb at dilution of 1:50 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded mouse heart using RPL8 Rabbit pAb at dilution of 1:50 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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400-660-9091

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com